Stillman Research Group (November, 2013)

Research Interests

Arsenic Binding to Metalloproteins

Metal Binding Motifs in Metallothioneins

Zinc Binding Motifs in Metallothioneins: the actual mechanism one Zn at a time

Electronic structures in Phthalocyanines and Porphyrins

Effects of Ring Oxidation and Reduction on the Spectral of Phthalocyanines and Porphyrins

Metal-induced Protein Folding

Iron Acquisition in Staphyloccocus aureus (the Isd Pathway)

Theoretical Aspects of Porphyrinoid Spectroscopy

Our techniques and methods

Metalloprotein metallation studies

Over-expression of metalloproteins

Extensive use of time- and temperature-dependent electrospray mass spectrometry

Use of absorption, emission, CD, magnetic CD, NMR, and X-ray spectroscopy, together with stopped-flow-methods

Modelling studies of protein metallation and folding

Our theoretical studies of porphyrinoids uses DFT methods

Electrochemical studies of porphyrinoids for cation and anion radicals

Theoretical Aspects of Porphyrinoid Spectroscopy uses DFT methods

Theoretical Aspects of Porphyrinoid S pectroscopy uses DFT methods

Links of Interest

University of Western Ontario

UWO Chemistry Department

Studying at Western

City of London

Research on Bioinorganic Chemistry at Western

Join us for 4th year work (both in Chemistry and Biology), Graduate Work and Summer Research with ICE and USRA funding

We welcome you to join our group. Western is really nice place to work at. The campus is beautiful and Chemistry is fully equipped. Living is easy and possible very close to campus. The Stillman lab has one of everything we need - complete recombinant protein production facility; new spectrometers - optical (UV-visible, CD, MCD, emission, and our beautiful micrOTOF II ESI-MS ... please continue at the end of this column... (for some reason we have lost that paragraph..).

News


Looking far ahead to May, 2015 and CanBIC-5

CanBIC-5 is ready to go. The symposium leaders should be busy finding their speakers, the www.canbic.ca web site is nearly refurbished - the symposia are updated, the dates are correct. Soon the site will be ready. We launch a facebook page: www.facebook.com/canbic for photos and chat about the conference but www.canbic.ca remains the source for technical information. We will note here when new informaiton has been uploaded to canbic.ca. We hope to see you in Parry Sound in May, 2015.


Looking ahead to June, 2014

Yes, the blog was supposed to be a monthly report but June will be busy:
First the CSC Conference in Vancouver - papers with Tyler and Gordon, and Martin's Alcan address. Then ICPP-8 in Istanbul at the end of the month where we will meet with so many old friends. At the end of that conference a number of the speakers will travel to Technion in Tel Aviv for a short workshop arranged by long time CanBIC supporter Zeev Gross; so nice to be able to be at his home university.



April, 2014

The 2013-2014 teaching year draws to a close with the 4th Year Research Day on the 5th April (see the "chemistryatwestern" facebook page for the programme and photos from the event. We congratulate Brit Lewis and Daisy Wong for an outstanding research year on their projects. Brit Lewis won 1st prize in the Inorganic session at SOUSCC (see fb pages) for her paper on the Zn-binding mechanism to MT - Congratulations!

The summer students (Lauren, Matt, and Tiffany) will join Daisy, Gordon and Tyler starting on the 1st May for an exciting 4 months of summer research. Martin travels to Europe on the 10th April and will visit researchers in Germany to establish a new collaborative project for the future.
We received outstanding news that each of the students in the group has been awarded scholarship support for their next studies. Metallothionein has been kind to us all it seems.


January-March, 2014

The Winter was terrible. It snowed very often and there were more days with highs below -10 C than I can remember over the last 30 years in London - known as part of the 'banana belt'! Only frozen banana milk slushies are possible from this winter. A review long in progress finally was published in Metallomics on work from Duncan Sutherland's PhD Thesis that was approved in April, 2012. Work from Mike Tiedemann studying the artificial proteins of prof Ron Koder also was published. Our first paper with Taka Kohzuma's group in Mito, Japan, Aki's work on cyt c' has finally been submitted.

 



Recent papers

Sutherland, D.E.K.; Stillman, M.J.(2014) "Challenging Conventional Wisdom: Single Domain Metallothioneins", Metallomics DOI: 10.1039/C3MT00216K
Mack, J., Otaki, T., Durfee, W.S., Kobayashi, N., Stillman, M.J. (2014) "MCD spectroscopy and TD-DFT calculations of low symmetry subnaphthalocyanine analogues", J. Inorg. Biochem. DOI:10.1016/j.jinorgbio.2014.01.001
Mutter, A.C.; Norman, J.A.; Tiedemann, M.T.;(2014) Singh, S.; Sha, S.; Morsi, S.; Ahmed, I.; Stillman, M.J.; Koder, R.L. “Rational design of a zinc phthalocyanine binding protein”, J. Structural Biology,185, 178-185.
Irvine, G.W.; Stillman, M.J. (2013) “Topographical analysis of As-induced folding of alpha-MT1a.”. Biochem. Biophys. Res. Commun. 441, 208-213. doi: 10.1016/j.bbrc.2013.10.044.

Fall in 2013

August, 2013 to 2014
We welcomed prof Chris Levy to the group from Kansas State University. Chris will spend the year with us while on Sabbatical. He is focusing on continuing Maysaa's 4th year project from 2012-2013 and trying his hand at porphyrin characterization following Brit's summer work on Bernhard Krautler's compounds in the 2013 summer.
Martin travelled to China in December, meeting up with colleague prof Zhen Shen in Nanjing, attending AsBIC in Guangzhou and then travelling to Beijing to give a talk on metallothioneins at Peking University hosted by prof. Jun-Long Zhang. He also met up with daughter Jacqueline and her husband Matthew Kalisz who work at the Canadian Embassy.
In early November, Martin gave a research lecture at Ryerson University and then the next week the group participated at the Inorganic Discussion Weekend held at York University. We took advantage of travelling to Toronto to meet and discuss work with prof Gideon Wolfaardt - Lindsay Jackson's PhD supervisor (see below - Lindsay was a UWO Biology student who worked with us on MT in 2010).
It is announced that Martin has won the 2014 Rio Tinto Alcan Award of the Canadian Society for Chemistry recognizing his bioinorganic research. He will deliver the award lecture in Vancouver at the Annual CSC Conference in June. See the notes for May, 2014 for the titles of the papers submitted to the CSC Conference (papers authored by Tyler Pinter and Gordon Irvine will be submitted).

July, 2013

We enter July with research picking up speed - we welcome back Dr Duncan Sutherland who will work with us to improve our expression yields for each of our protein constructs. he will also be working on a review based on his Ph D thesis of last year.
With the summer here, Martin starts to travel again - going to the UK to meet with Dr. Peter Kille, the founder of our constructs - to discuss the expansion of our zinc work towards the homeostatic control of zinc and copper, our interest for the last two years. Martin then travels on to South Africa to give a series of lectures before returning at the beginning of August.

We've noticed a formatting error below - will be fixed asap!

At Rhodes University in South Africa there will be an airing of a new version of "Green blood". The paper with Ron Koder is at the proof stage - thanks Mike - and Tyler is telling the world about his carbonic anhydrase work in Grenoble - brand new ideas that will change the world - brilliant!


June, 2013

The CanBIC-4 Conference passed - and by all accounts was more successful than previous editions! We were lucky that cool and damp weather of the beginning of the week transformed into glorious days on the Friday and then for the Saturday Georgian Bay Day at Killbear Provincial Park.

Please see the series of photos on the group facebook page:
"stillmanbioinorganicgroup"
Interesting blogs from the New Journal of Chemistry editor also highlight the CanBIC conference and Georgian Bay Day, please see:

http://blogs.rsc.org/nj/2013/05/30/njc-at-canbic-4-in-ontario/
http://blogs.rsc.org/nj/2013/06/11/georgian-bay-day-at-killbear-park/

 

 

 


To students interested in the role of metals in biology, our group answers difficult but biologically relevant questions by applying many techniques to build up an imperfect but extensive picture of the biologically-significant metal-based chemistry. Please contact us for both 4th year (in Biology and Chemistry) and graduate research (in Biology and Chemistry). Currently we are very interested in expanding our work with zinc and copper, especially cellular interactions. The work with hemes and porphyrins is also very active at the moment and we are keen to expand our work in the prediction of the colour of these dyes.

We welcome Brad Reed from the dept. of Biology who will work on metal binding to a seaweed MT.  


May, 2013

The CanBIC-4 Conference is just a week away - everybody in the group is participating in this important Canadian event. The full scientific programme and many other details, including Music at Canbic and pictures from the 2011 CanBIC-3 Georgian Bay Day, can now be found on the updated web site: www.canbic.ca.
CanBIC has become the meeting place for bioinorganic chemistry in Canada with participants in many of the most important areas of bioinorganic chemistry world-wide meeting to discuss current projects.

For us, the metallothionein symposium offers a summary of current knowledge on metallothionein, Tyler and Martin contribute papers. Gordon contributes a poster on his work with cysteine modification. Maria will talk on NOS from her NIA research. Tasha and Brit have teamed up to present a poster on Bernhardt Krautler's porphyrins (Brit is really working on the CdMT binding mechanism question but used her down time while protein was growing to push out the porphyrin data). As for the previous CanBIC conferences, the coming together of so many experts in this field to Parry Sound, is a boost to our own work and the feast is about to happen. Of course, we meet our old friends and exchange news and greet new friends and colleagues. We hope all who consider themselves to be bioinorganic chemists take advantage of this remarkable opportunity.

After CanBIC comes the Metallomics Symposium and the Bioinorganic Symposium at the CSC 2013 in Quebec City. We have talks and posters planned for those symposia. That will leave us at the end of May with many projects underway for the rest of the summer.


To students interested in the role of metals in biology, our group answers difficult but biologically relevant questions by applying many techniques to build up an imperfect but extensive picture of the biologically-significant metal-based chemistry. Please contact us for both 4th year (in Biology and Chemistry) and graduate research (in Biology and Chemistry). Currently we are very interested in expanding our work with zinc and copper, especially cellular interactions. The work with hemes and porphyrins is also very active at the moment and we are keen to expand our work in the prediction of the colour of these dyes.

We welcome Brittany Lewis who will work on Cd binding to MT as we have a gap in our research plans (but she was distracted by the porphyrin chemistry of Bernhardt Krautler first studied by summer student Tasha Jarisz from U. Victoria - ICE scholar in 2012.) We will report our results as a poster at CanBIC then as a paper. We welcome any students to come to work on the metallation of MT projects that are outstanding. We welcome Jasjit Singh who is working on the metallation sequence in metallothionein - Jasjit is an Inorganic Chemistry Exchange scholar for 2013 from Ryerson University in Toronto.  


April, 2013

Congratulations to Tyler for winning travel funds to attend ICBIC in Grenoble in July.


Congratulations go to all the graduates in the group for being awarded research scholarships to support their work starting on May 1st! We welcome three summer students who will work on our metallothionein projects and will also help with the CanBIC conference. Abstracts for CanBIC-4 are arriving now and the programme is filling up.  Over 135 registrations received.  The May 21st start for CanBIC is very close now. We sadly say goodbye to Dr Michael Tiedemann on his way to Dental School - but where? (He will be back at CanBIC though.)  www.canbic.ca

March, 2013

We will see what comes in March, there is much uncertainty.  Preparations for CanBIC-4 are underway for a May 21st starting date.  We have put several papers into the Canadian Society for Chemistry Annual meeting that will be held in Quebec City the following week.  Martin is co-chair of the Metallomics Symposium and a well we have contributions by Martin and Maria in the Bioinorganic Symposium as well as posters from the group.  A busy start to the summer.

A really important paper about zinc binding to metallothionein is published on the Biochemistry web:

Summers, K.L.; Sutherland, D.E.K.; Stillman, M.J. (2013)Biochemistry, 52, 2461-2471. "Single-Domain Metallothioneins: Evidence of the Onset of Clustered Metal Binding Domains in Zn-rhMT 1a",  Biochemistry.  DOI: 10.1021/bi400021b

Maysaa Assaf takes her project talk about Mn(II) binding to MT to SOUSCC at McMaster University on Saturday, 30th March.

 

February, 2013

Gordon Irvine’s first paper is accepted.

Gordon W. Irvine, G.W.; Summers, K.L.; Stillman, M.J. (2013) “Cysteine accessibility during As3+ metalation of the alpha and beta-domains of recombinant human MT1a.”. Biochem. Biophys. Res. Commun., 433, 477-483.

 

January, 2013

Started well with a review on AgMT finally being published in the Encyclopedia of Metalloproteins and with us welcoming Maria Salgado back to the group.

Summers, K.L. and Stillman, M.J. (2012) "Silver Metallothionein" in Encyclopedia of Metalloproteins (Kretsinger, Robert H.; Uversky, Vladimir N.; Permyakov, Eugene A. (Eds.), Springer (2013).

Dr Maria Salgado Banegas joined us to work on metallothioneins.  Maria was last at the National Institute for Aging in Baltimore, Maryland where she was investigating the effects of NO on cardiovascular issues.

December, 2012

The group was very busy this year and we published nine really wonderful papers

Sutherland, D.E.K.; Summers, K. L.; Stillman M. J. (2012) "Modeling the Zn2+ and Cd2+metalation mechanism in mammalian metallothionein 1a" Biochem. Biophys. Res. Commun. 426, 601-607.

Summers K. L.; Mahrok A.K.; Dryden M.D.M.; Stillman M.J. (2012) "Structural properties of metal-free apometallothioneins" Biochem. Biophys. Res. Commun. 425: 485-492.

Tiedemann, M.; Heinrichs, D.E.; Stillman, M.J. (2012) “The multi-protein heme shuttle pathway in Staphylococcus aureus: Isd cog-wheel kinetics”, J. Am. Chem. Soc. 134, 16578-16585.

Tiedemann, M.T.; Pinter, T.B.J.; Stillman, M.J. (2012) "Insight into blocking heme transfer by exploiting molecular interactions in the core Isd heme transporters IsdA-NEAT, IsdC-NEAT and IsdE of Staphylococcus aureus", Metallomics, 4, 751-760.  DOI: 10.1039/C2MT20067H  (featured on cover). (TOP 10 downloads in July, 2012)

Sutherland, D.E.K.; Summers, K.L; Stillman, M.J. (2012) "Noncooperative metalation of metallothionein 1a and its isolated domains", Biochemistry, Biochemistry 51: 6690-6700.

Bohle, D.S.; Dodd, E.L.;Pinter, T.B.J.; Stillman, M.J. (2012) "Soluble diamagnetic model for malaria pigment: coordination chemistry of gallium(III) protoporphyrin-IX", Inorg. Chem., 51, 10747-10761.

Tiedemann, M.T.; Stillman, M.J. (2012) "Heme binding to the IsdE(M78A; H229A) double mutant: Challenging unidirectional heme transfer in the Iron Regulated Surface Determinant (Isd) protein heme transfer pathway of Staphylococcus aureus", J Biol Inorg Chem; 2012 Oct;17(7):995-1007.

Pinter, T.B.J.; Dodd, E.L.; Bohle, D.S.; Stillman, M.J. (2012) "Spectroscopic and Theoretical Studies of Ga(III)protoporphyrin-IX and Its Reactions with Myoglobin", Inorg. Chem. 51, 3743-3753.

Sutherland, D.E.K.; Willans, M.J.; Stillman, M.J. (2012) "Single domain metallothioneins: Supermetalation of human MT 1a", J. Am. Chem. Soc., 134, 3290-3299.

 

And two book chapters also were published

Ngu, T.T. and Stillman, M.J. (2011) “Metallation of seaweed Fucus vesiculosus metallothionein:  As3+ and Cd2+ binding” in Handbook of Marine Macroalgae: Biotechnology and Applied Phycology”, ed. Se-Kwon Kim, Wiley, Chichester, UK, Ch. 14., pp 302 – 320.

Sutherland, D.E.K., and Stillman, M.J. (2012) “Metallothioneins” in “"Brain Diseases and Metalloproteins", edited by David R. Brown, Pan Stanford Publishing, Singapore, in press, Ch. 4, pp 1-58. ISBN: 9789814316019

 

And the group gave a large number of Conference papers as well as talks by Martin to Universities.

 

"Theoretical and spectroscopic advances in the interpretation of the electronic spectra of porphyrins and phthalocyanines", ICPP-7, Korea, 1-7 July, 2012.

"Understanding the green of leaves- spectroscopic and theoretical studies", Ibaraki University, Mito, Japan, 29th June, 2012

"Metalation pathways in metallothionein elucidated with time- and temperature-resolved ESI-mass spectrometry", Canadian Chemistry Society National Conference, 26-30 May, 2012, Calgary, Alberta.

"Relating spectral properties of porphyrins and phthalocyanines to theoretical parameters from their electronic structures", Martin Stillman, Lydia Kwan, Kyle Jeffs, Rohit Deshpande, Yongming Deng, Lin Jiang, and Hong Wang,  Canadian Chemistry Society National Conference, 26-30 May, 2012, Calgary, Alberta.Canadian Chemistry Society National Conference,

"Relating spectral properties of porphyrins and phthalocyanines to theoretical parameters from their electronic structures".  Martin Stillman, Lydia Kwan, Kyle Jeffs, Rohit Deshpande, Yongming Deng, Lin Jiang,and Hong Wang, 12th EurAsia Conf. on Chemical Sciences, 16-21 April, 2012 Corfu, Greece.

" Metalation pathways in metallothionein elucidated with time- and temperature-resolved ESI-mass spectrometry", Dept. of Biology, The University of Western Ontario, 28th March, 2012.

 

 

November, 2012

A very busy month.  Martin flies to Hong Kong to give an invited lecture as AsBIC-VI and then a lecture at the Chinese University of Hong Kong.  A quick flight back (15 hours just to Toronto) and straight into teaching Monday’s Chem 4441A class.  Not very restful!

 

September, 2012

Gordon Irvine joins the group for graduate work studying the kinetics of metal binding.  Gordon finished his BSc at Brock University.

Maysaa Ali Assaf joined the group for her 4th year research project (Chem 4491E)

Martin travels to Grenoble to participate in the IMBG BioInorganic Conference.  

 

August, 2012

Duncan Sutherland leaves to go to UWO’s Medical School.  

May, 2012

Both Mike Tiedemann and Duncan Sutherland did submit their PhD theses on time and are now fully fledged “Dr’s”.

They both continued to work in the lab to finish up projects.

January, 2012

The start of a busy year because both Mike Tiedemann and Duncan Sutherland will submit their PhD theses before May 1st.

December, 2011

We published four papers this year and a book chapter.  But wait for 2012!

 

Kaluarachchi, H.; Siebel, J.; Kaluarachchi-Duffy, S.; Krecisz, S.; Sutherland, D.; Stillman, M.J.; Zamble, D.B. (2011) “The in vitro selectivity and in vivo specificity of the E. coli nickel metallochaperone, SlyD”, Biochemistry, 50, 10666-10677.

Tiedemann, M.T.; Stillman, M.J. (2011) “Magnetic circular dichroism studies of porphyrins”, J. Porphyrins Phthalocyanines, 15, 1134-1149.

Xue ZL, Mack J, Lu H, Zhang L, You XZ, Kuzuhara D, Stillman M, Yamada H, Yamauchi S, Kobayashi N, Shen Z. (2011) The synthesis and properties of free-base [14]triphyrin(2.1.1) compounds and the formation of subporphyrinoid metal complexes.  Chemistry Eur. J., 17, 4396-4407.

Sutherland, D.E.K.; Stillman, M.J. (2011) “The magic numbers of metallothionein”, Metallomics, 3, 444-463.  (Featured on the cover; Top 10 downloads in 2011).

 

November, 2011

No IDW this year because it was held in August as part of the silicon conference. Martin travels to Taka Kohzuma's lab for a week to finish writing papers from the June experiments (Ibaraki University in Mito, Japan). A quick visit as the teaching term continues.

October, 2011

A quiet month as classes continue. Janice Lee, another old-group member, who isn't - came and visited the lab on the eve of Western's Homecoming. The Thanksgiving Weekend was beautifully warm - photos to come- in Parry Sound.
Martin will give a talk "If grass is green, why isn't blood green?" as part of "The International Year of Chemistry during National Chemistry week" in Philadelphia at Drexel University.
Our chapter in the Handbook of Marine Macroalgae: Biotechnology and Applied Phycology”, is published: Ngu, T.T. and Stillman, M.J. (2011) “Metallation of seaweed Fucus vesiculosus metallothionein: As3+ and Cd2+ binding”. Book edited by prof. Se-Kwon Kim, Wiley, Chichester, UK, Ch. 14., pp 302 – 320.

September, 2011

Classes start again. Martin is teaching Chemistry 4441a - the "Advanced structure and bonding" course. Kyle Jeffs joins the group for his 4th year research project "discovering the optical and theoretical properties of a range of push-pull porphyrins', this is a continuation of Lydia Kwan's project. A collaboration with prof. Hong Wang at Miami University in Oxford, Ohio.

August, 2011

Martin and Duncan participate in ICBIC-13 in Vancouver, spending a week in Vancouver. It doesn't rain! We meet up with old-group member Jayna Chan (well, past-group member - she's not that old!).
Anjan and Martin travel to Montreal for the annual ICE Workshop held at the University of Montreal hosted by Garry Hanan. Photos from ICBIC and Montreal at: www.stillmanbioinorganicconfs2011.shutterfly.com
This brings us to the end of the summer.
Martin moves to Georgian Bay to watch the ducks and prepare for the Fall term.

July, 2011

Martin travels to Nanjing to work in the laboratory of prof. Zhen Shen. He visits the Terracotta Warriors in Xi'An and participates with Zhen Shen and Nagao Kobayashi in the Nanjing Bioinorganic Workshop.
Martin spends a day at Fudan University in Shanghai as a guest of profs. Tao Yi and Fuyou Li and is taken round the Shanghai Urban Museum - a must-see museum in the People's Square. The Air Canada flight is 8 hours late leaving so after the museum he takes a ferry across the Yangtze River to watch sunset over Shanghai. Highly recommended. Pictures at www.stillmanbioinorganicconfs2011.shutterfly.com - check out 'visits to colleagues' labs' section.

June, 2011

CanBIC is over - by all accounts better than -1 in 2007 and -2 in 2009. Beautiful weather. A crammed full programme - find the pictures on shutterfly at:
www.stillmanbioinorganicconfs2011.shutterfly.com

Martin travels to Montreal to take part in the Bioinorganic Symposium at the National Chemical Society of Canada Conference. The symposium organized by Scott Bohle of McGill and featered many CanBIC speakers. Was very nice.
We welcome three students from prof. Taka Kohzuma's lab at Ibaraki University in Mito, Japan.
Tomonori Muroya, Takahide Yamaguchi, and Akiko Takashina. A short report on the Western Faculty of Science News site: www.uwo.ca/sci/news/japan_students.html.

May, 2011

May is filled with preparation for CanBIC and with Lydia graduating.
Also, we welcome Anjan Mahrok from University of Calgary for summer work. Anjan is an ICE Award winner as well as a USRA winner. She is working on BQ binding to MT. We welcome Daisy Wong who after helping with CanBIC will join Duncan Sutherland to study the interactions of some drug molecules with MT - this continues work of Lindsay Jackson, who is now a PhD student at Ryerson University in Toronto.
Our paper in collaboration with prof. Zhen Shen in Nanjing is published:
The synthesis and properties of free-base [14]triphyrin(2.1.1) compounds and the formation of subporphyrinoid metal complexes. Xue ZL, Mack J, Lu H, Zhang L, You XZ, Kuzuhara D, Stillman M, Yamada H, Yamauchi S, Kobayashi N, Shen Z., Chemistry. 2011, 17:4396-407.

April, 2011

The CanBIC conference is only in little over a month now. Over 155 participants. A very full programme. Please go to www.canbic.ca for details takes shape. By the 24th Feb., over 100 registrations had been received. The Local Organizing Committee is hard at work: Mike Tiedemann, Duncan Sutherland, Tyler Pinter, Daisy Wong, Tom Stockman, Kalen Swanick and David Stillman. The International Organizing Committee is Martin Stillman, Nagao Kobayashi (Tohoku) and Frank Shaw (Illinois State).
Katie Stillman and Angela Park will provide the music for "Music at CanBIC" during the conference. The participation rate for the conference is ahead of 2009 so it looks like 2011 will be the biggest yet. Join us: May 31st - June 4th in Parry Sound (www.canbic.ca).
“The magic numbers of metallothionein”, is published in Metallomics (Sutherland, D.E.K.; Stillman, M.J. (2011) Metallomics, 3, 444-463) The image of Cd8-MT is featured on the front cover.
pubs.rsc.org/en/content/articlepdf/2011/mt/c1mt90018h/2011-05-01?

February, 2011

Reading week has been busy - Lydia's experiments are rolling along with successes at every step: amazing ESI-MS data from the Wang complexes; wonderful MCD data and even the calculations are working.

The CanBIC conference takes shape. By the 24th Feb., over 100 registrations had been received. 86 Invited Speakers had accepted our invitations. The Local Organizing Committee started work: Mike Tiedemann, Duncan Sutherland, Tyler Pinter, Daisy Wong, Tom Stockman, Kalen Swanick and David Stillman.
The International Organizing Committee is Martin Stillman, Nagao Kobayashi (Tohoku) and Frank Shaw (Illinois State). Katie Stillman and Angela Park will provide the music for "Music at CanBIC" during the conference. The participation rate for the conference is ahead of 2009 so it looks like 2011 will be the biggest yet. Join us: May 31st - June 4th in Parry Sound (www.canbic.ca). Metallomics accepts our invited critical review: "The Magic Numbers of Metallothionein", by Duncan Sutherland and Martin Stillman.

January, 2011

Martin is teaching "Inorganic macromolecular complexes (MMCs): Advanced structure and bonding", Chem 4441b.

This month was a busy one for new people. Daisy Wong has joined us to help with the CanBIC conference and then work during the summer on Nikosi's phthalocyanine and MT project. Anjan Mahrok wins a USRA scholarship to study in MJS' lab.

December, 2010

Ms. Anjan Mahrok at the University of Calgary is awarded an Inorganic Chemistry Exchange scholarship (ICE) to work in our lab in the 2011 summer.

The Chemistry 2211a Posters are outstanding as usual. Christmas brings terrible weather.

October, 2010

Summer 2011 projects are available; a new instrument is installed; Fall is really here.

We welcome enquiries for summer 2011 projects from high achieving students in the ICE and MITAC-global programmes.

The Stillman lab offers a large number of exciting summer-long research projects for 2011 in bioninorganic chemistry. Western is a great university to come to - living here is really pleasant in the summer and costs are low. We will train you to use our new state-of-the-art instrumentation and theoretical methods. Current on-going projects involve characterization of novel porphyrins, studies of metallation to metallothioneins, studies of metal exchange between proteins, and studies of the heme-binding proteins in bacteria. We have projects available from metal binding to a range of proteins using ESI-mass spectrometry to porphyrin spectroscopy coupled to theoretical calculations. Please contact Prof. Stillman for more example (martin.stillman@uwo.ca). We look forward to hearing from you.
Our new Bruker micrOTOF-II time-of-flight mass spectrometer is installed - providing enhanced ESI-MS capabilities and much greater sensitivity and resolution. First data looking excellent.

September, 2010

Classes start, new 4th year researchers.

Martin gives an invited lecture at the Japanese Biophysical Society Annual Meeting in Sendai travelling with colleague Prof. Kohzuma from Mito. Martin is teaching "Metals in Life", Chemistry 2211a.


We welcome 4th year students to consider research in our lab on one of the many different topics available - see the 4490 link.

Martin starts teaching Chemistry 2211a "Metals in Life"

Tyler Pinter joins as a new graduate student

Lydia Kwan joins the group for her 4th year research project; Lydia is working on characterizing novel porphyrins from the labs of Drs. Hong Wang (Ohio) and Berhard Krautler (Austria).

Martin gives an invited lecture at the Annual Meeting of the Biophysical Soc of Japan, Sendai, Japan, "Metalloprotein conformations by ESI-mass spectrometry"

August, 2010

Finishing up projects & Mike Dryden and Martin travel to the ICE Workshop in Winnipeg
ICE Workshop, 19-20 August in Winnipeg: Mike Dryden presents a summary of his work on As-MT and Martin presents a lecture "Why isn't blood green".

July, 2010

ICPP-6 in New Mexico

Martin and Mike Tiedemann participate in ICPP-6. Martin is co-organizer of symposia on "Theoretical Methods in Porphyrinoids" (25) and "Spectroscopy in Heme Proteins" (18) and gives an invited talk about Isd proteins. Mike presents a poster on Isd proteins.

June, 2010

Canadian Society of Chemistry National Conference in Toronto
Martin gives a lecture on Metallothioneins in the Bioinorganic Symposium organised by Deborah Zamble (U of T).

May, 2010

A New Summer Research Starts: New faces - New projects

Mike Dryden joins the group as an Inorganic Chemistry Exchange (ICE) scholarship winner to work on As-metallothioneins.

Tyler Pinter joins to carry out summer research on the Isd system.

Lindsay Jackson joins to finish up her work with the RAPTA compounds in collaboration with Angela Casini at EPFL, Lausanne.

April, 2010

Martin gives a lecture in Hong Kong, China

Mechanism of Arsenic Binding to Metallothioneins from Time -and Temperature-Resolved Electrospray Ionization Mass Spectrometry. Unravelling the complete metal binding mechanism for a multiple-metal binding protein, on April, 27th.

Martin gives a lecture at the Sun Yat-Sen University, Guangzhou, China

Why isn't grass red and blood green? Understanding spectra of macrocyclic compounds: linking absorption and magnetic circular dichroism data of porphyrinoids with theoretical calculations, on April, 25th.

Martin gives lecture at Fudan University, Shanghai, China

Understanding iron acquisition via the Isd proteins in the human pathogen Staphylococcus aureus - studies using magnetic circular dichroism spectroscopy and mass spectrometry. Can we beat the super bug MRSA this way? April, 22nd.

Martin gives lecture in Hangzhou, China

Mechanism of Arsenic Binding to Metallothioneins from Time -and Temperature-Resolved Electrospray Ionization Mass Spectrometry. Unravelling the complete metal binding mechanism for a multiple-metal binding protein, on April, 19th.

Martin gives lecture in Nanjing, China

Why isn't grass red and blood green? Understanding spectra of macrocyclic compounds: linking absorption and magnetic circular dichroism data of porphyrinoids with theoretical calculations, on April, 14th.

Martin gives lecture in Beijing, China

Why isn't grass red and blood green? Understanding spectra of macrocyclic compounds: linking absorption and magnetic circular dichroism data of porphyrinoids with theoretical calculations, on April, 10th.

Martin will visit China for a tour of research labs

Martin will talk on Porphyrins and Metallothioneins in Beijing, Nanjing, Shanghai and Hong Kong

Talk to IBMB at Waterloo

Martin's talk is on the kinetics of As(III) binding to metallothioneins; 3:30 pm on April, 6th.

March, 2010

4th year conference at UWO and 4490-Day

Each of the 4th year students (Sandra, Kristen, Arthur, Tyler) presented talks on their research successes at the annual 4th year research conference held at Western on Saturday, 20th March.

February, 2010

Duncan Sutherland attends Metals in Biology Gordon Conference

Duncan travels to California to attend the Bioinorganic Chemistry Gordon Research Seminar

Martin visits Grenoble & Lausanne

Martin took part in a site visit of the Metals in Biology Research Centre in Grenoble. He also visited EPFL at Lausanne to discuss recent results from Lindsay with Angela Casini and Paul Dyson; finally visits Steve Sturzenbaum at King's College before flying home for the UWO reading Week.

January, 2010

Ibaraki Univ for UV Res Raman and then to Tohoku Univ

Martin travels to Mito in Japan to visit Prof Taka Kohzuma to try out a new experiment on the Cd-clusters in MT with his group. After this Martin travels with Nagao Kobayashi to Ueda and then to Sendai to work in Nagao's lab for 2 weeks.

December, 2009.

End of term and Christmas break

November, 2009.

Visit to Europe

Martin travels to labs in Eindhoven (Merkx), Zurich (Freisinger), Lausanne (Casini & Dyson), King's (Sturzenbaum) and Warwick (Blindauer & Sadler) to give talks and discuss collaborative projects. Martin and Claudia Blindauer run a week-long workshop on Bioinorganic Methods and Ideas in Warwick - "Metals in Life's Processes".

October, 2009.

Nothing happened in October!

September, 2009.

A New Year: New faces - New projects

Tyler Pinter, Arthur Yu, Kristen King and Sandra Krecisz join for their 4th year research projects.

Lindsay Jackson (in 4th year Biology) has joined the group as a research assistant to work on metal binding to metallothioneins. See 2010 for report of her successes with compounds from EPFL.

July, 2009.

ICBIC 14 in Nagoya, Japan

The group travels to Japan.

Martin Stillman, Mike Tiedemann and Duncan Sutherland all travel to Japan to attend ICBIC-14 in Nagoya. Martin gives lectures at the Ibaraki University in MTO (Isd studies), and Tohoku University in Sendai (Isd studies), then at ICBC-14 (As-MT ESI-MS studies) and finally at the post-ICBIC Mito Bioinorganic Symposium (As-MT ESI-MS studies).
Duncan and Mike present posters at ICBIC-14.

June, 2009.

Thanh officially graduates with her Ph.D.

May, 2009.

The Stillman group members organize the 2nd Georgian Bay International Conference on Bioinorganic Chemistry in Parry Sound

Martin is the Conference Chair, with Nagao Kobayashi and Frank Shaw as the organizing committee
John, Duncan, Mike, Janice, Tyler, Carly, Susan H?, David Stillman are the local organizing committee

Our group makes several presentations and posters
Tiedemann, M.T., Muryoi, N., Heinrichs, D.E., Stillman, M.J. “Heme Transfer Pathway in Staphylococcus aureus using Electrospray Ionization Mass Spectrometry and Magnetic Circular Dichroism spectroscopy.”
Hewson, C. J.; Tiedemann, M. T.; Stillman, M. J. “Spectroscopic Properties of Myoglobin as a Function of Axial Ligand.” 
Lee, J.A.; Ngu, T.T.; Rushton, M.K.; Stillman, M.J. “The Arsenic Metallation Pathway in Seaweed Fucus Vesiculosus Metallothionein.” 
Pinter, T.B.J.; Lee, J.A.; Ngu, T.T.; Stillman, M.J. “Domain Dependence of the Arsenic Metalation of Metallothionein.”
Sutherland, D.E.K., Stillman, M.J. “Metallation properties of alpha and beta human metallothioneins.” May, 2009.

Carly Hewson and Tyler Pinter join the group as summer students

April, 2009.

Duncan and Mike receive travel scholarships to attend ICBIC-14 in Nagoya

February, 2009.

Martin goes to South Africa to give two talks

To the Joint JSPS/NRF South Africa Workshop: “Spectroscopic and theoretical studies towards answering the question: Why isn’t grass red and blood green?” 

To the Department of Chemistry, University of Cape Town: “Mechanism of Arsenic(III) binding to Metallothioneins: Time- and temperature-dependent kinetic studies using electrospray mass spectrometry”.

November, 2009.

Lee joins the group as a research assistant

The group participates in the Inorganic Discussion Weekend at Brock University

September, 2009.

Martin is a Topic Lecturer at the European Chemical Congress in Turin       

Martin gives an invited talk: “Metal binding mechanisms in metallothioneins”. September, 2008.

Duncan is awarded a travel scholarship to attend the Tohoku University Summer School.
Duncan gives a talk entitled “Metal thiolate cluster motifs in the protein metallothionein”, to the Tohoku University Global Center of Excellence (COE) Summer School, Sendai, Japan.

July, 2008.

Martin and Mike travel to Moscow for ICPP-5
Martin gives an invited lecture "Theoretical and spectroscopic advances in the interpretation of the electronic spectra of porphyrins and phthalocyanines"
John Mack, coauthored with Martin and Nagao Kobayashi, gives an invited talk: "MCD spectroscopy and the effect of porphyrinoid ligand non-planarity: core modified tetrabenzoporphyrins”
Mike presents a poster.

January, 2008.

Michael Tiedemann wins scholarshipstrong>

Michael Tiedemann has been awarded a Graduate Thesis Research Award which helps to provide travel funds to attend and present his work at ICPP-5 in Moscow in July, 2008.

Thanh Ngu Heads to London, England for 3 weeks research with Stephen Sturzenbaum's group!

Thanh Ngu is spending 3 weeks in King's College, London, with Stephen Sturzenbaum working on metallothionein (she comes back after a week in Paris!)  Thanh's travel was supported by an ASPIRE Award.

June, 2008.

Biochemical Society Focused Meeting, Norwich, UKstrong>

Martin Stillman was invited to speak at the Biochemical Society Focused Meeting entitled Transition Metals in Biochemistry in June, 2008.

Martin speaks at his old University on the occasion of Andrew Thomson's retirement
“Understanding iron acquisition in Staphylococcus aureus using MCD spectroscopy and mass spectrometry”.

Martin travels to Alberta to speak at the University of Calgary and 92nd Canadian Chemical Conference
“Understanding geometrical control of the colours of porphyrins – understanding novel magnetic circular dichroism spectroscopic spectra of a saddled porphyrin.  Or, why isn’t our blood green and grass red?”
 “Understanding iron acquisition in Staphylococcus aureus using MCD spectroscopy and mass spectrometry”  in the  “Symposium on Bioinorganic Chemistry” 
“The Mechanism of Arsenic Binding to Metallothioneins from Time and Temperature-Resolved ESI-MS”  in the  “Symposium on Bioinorganic Chemistry”

Stillmangroup at IDW 2007!

The Stillman Group went to The University of Toronto for the Inorganic Discussion Weekend conference (IDW). Photos can be found here!

Welcome New Group Members!

We welcome two new graduate students: Michael Tiedemann and Duncan Sutherland. We also welcome our two undergraduate thesis (490 Project) students: Meredith Gullons and Alex Easton! For more information please see the people section.

1st Georgian Bay International Conference

The 1st Georgian Bay International Conference on Bioinorganic Chemistry will be held in and around the Parry Sound area in May 2007. For more information please see the conference website.

A few photos

2009 and 2010

We have moved to Shutterfy.com -
The first album is for 2011

www.stillmanbioinorganicconfs2011.shutterfly.com
We will be going back in time slowly.
The CanBIC conference photos are at this location for now.

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More about working in our group...

    Joining our group for graduate studies, summer research or for your 4th year project

    If you are interested in joining the Stillman Bioinorganic Group for graduate school have a look here for more information about submitting an application. Research in our group offers a wide range of stimulating experiences at the boundary between inorganic chemistry and biology. Metals control life!


    Joining the Stillman Bioinorganic Research group, continued..... Join us for Graduate Work

    The Stillman lab has one of everything we need - complete recombinant protein production facility; new spectrometers - optical (UV-visible, CD, MCD, emission, atomic absorption, ESI-MS) and just this year a brand news Bruker micrOTOF II ESI-MS instrument; excited state lifetime capabilities from ns-millisec using our N2 laser, dyes and the doubler; low temperature measurement capabilities; electrochemistry and photochemistry facilities; access to as much computation as you want both in the lab in through our accounts on Sharcnet; a wonderful group of staff members helping us work. We collaborate with groups around the world with everybody in the lab travelling outside Canada most years to work in our lab or attend a conference - visits from both our collaborators and their students are frequent. In other words: please consider us for your graduate work. Oh yes, we are considered to be the happiest chemistry department in the country. We are.
    Current research topics available for new students: Studies of the metal binding kinetics in metallothioneins using optical, mass, and stopped flow techniques (see our papers on As(III)); studies of heme transport proteins; studies of the electronic structures of nonsymmetric porphyrins and corroles using MCD and theoretical methods.

    Fall 2014 projects are available

    We welcome enquiries for 2014-2015 projects from high achieving students in the ICE and MITAC-global programmes.

    The Stillman lab offers a large number of exciting summer-long research projects for 2014 in bioninorganic chemistry. Western is a great university to come to - living here is really pleasant in the summer and costs are low. We will train you to use our new state-of-the-art instrumentation and theoretical methods. Current on-going projects involve characterization of novel porphyrins, studies of metallation to metallothioneins, studies of metal exchange between proteins, and studies of the heme-binding proteins in bacteria. We have projects available from metal binding to a range of proteins using ESI-mass spectrometry to porphyrin spectroscopy coupled to theoretical calculations. Please contact Prof. Stillman for more example (martin.stillman@uwo.ca). We look forward to hearing from you.

 

 

 

Events

5th Georgian Bay Conference on Bioinorganic Chemistry

CanBIC-5 in 2015 (May 19th - 23rd, 2015 in Parry Sound.) Yes, Georgian Bay Day Kayaks will be available on Saturday 23rd May! Check out www.canbic.ca

ICPP-8

Conference July, 2014 in Istanbul, Turkey
We will be participating by organizing and presenting in the "Theoretical aspects of porphyrin spectroscopy" symposium.


CSC Conference

1st - 5th June 2014 in Vancouver

4th Georgian Bay Conference on Bioinorganic Chemistry

CanBIC-4 in 2013 (May 21st - 25th, 2013 in Parry Sound.) Georgian Bay Day Kayaks were wonderful - see the 'stillmanbioinorganicgroup' fb page for pictures! Check out www.canbic.ca

ICPP-7

Conference July 1-6, 2012 in Korea
We will be participating by organizing and presenting in the "Theoretical aspects of porphyrin spectroscopy" symposium.

CSC-2012

Conference May 26-30, 2012 in Calgary, Alberta.
We will be participating in the "Symposium to honour Barry Lever" and in the "Bioinorganic" symposium.

Eurasia-12

Conference April 16-21, 2012 in Corfu, Greece
We will be participating with a talk in the mini-symposium on aromatic nanomaterials.

3rd Georgian Bay Conference on Bioinorganic Chemistry

CanBIC (May 31st - June 3rd, 2011 in Parry Sound.)

 

ICPP 6

Conference July 4-9, 2010 in New Mexico, USA

CSC Conference

31st May - 2nd June 2010 in Toronto

2nd Georgian Bay Conference on Bioinorganic Chemistry

CanBIC (May 26-29 2009)

IDW 2008

Conference Nov 28-30 2008

ICPP 5

Conference July 7-11 2008

IDW 2007

Conference Nov 2-4 2007

1st Georgian Bay Conference on Bioinorganic Chemistry

CanBIC (May 22-25 2007)


 

Research Links